BD Fortessa. Print; back. Fortesa (standard configuration) Example of available markers: Emission filter (nm) Detector: Laser lilne/Color (Excitation) Indo-1 (blue

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Locate the green power button on the right hand side of the instrument; press to switch on the LSR-Fortessa. This will warm up the lasers and begin to pressurise the fluidic system. Some buttons on the fluidics panel, on the front of the instrument, should be illuminated indicating it is ON. 2. FORTESSA USER GUIDE COMPUTER SETUP: • Turn on computer before turning on the machine • Windows 10 Username: BDOperator • Password: BDIS#1 • Open Tera Term application on desktop and leave running in background • Log in to BD FACSDiva software MACHINE SETUP: • Turn on the machine if you are the first user of the day. There is no fluidics startup for the Fortessa.

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(Please refer to LSR Fortessa Standard Operation Protocol – Basic operation for Tube Mode) Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Immunoanalytics - Core Facility Geb. 90 Marchioninistr. 25 81377 München BD High Throughput Sampler User’s Guide For the BD LSRFortessa I. Introduction The BD High Throughput Sampler (HTS) is a high-speed sampling device used on the 5-Laser LSRFortessa located in Rm. 6006F of the RIA Division. The HTS provides users the ability to acquire samples from 96-well or 384-well plates for efficient analysis with The BD LSRFortessa has 16 detectors plus forward- and side-scatter detectors and an additional FSC PMT. The Fortessa is used for analysis only. The Fortessa can analyze up to 16 colors at one time. The Fortessa is also the instrument used for cell cycle, cell proliferation, cell membrane potential, and… BD LSRIIs and BD LSR-Fortessa have performance, capabilities, and flexibility never seen before in a benchtop analyzer.

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Disconnect the air line (green) and fluid line (Blue) from the sheath container. 3. Depressurize the sheath container by pulling up on the vent valve.

This core houses two cell sorters: a BD Influx (LSA) and a Beckman-Coulter Moflo legacy, and it also houses four analyzers: BD LSR II, BD LSR Fortessa(LSA ), 

This instrument can be run 24/7 by trained users as per our Policies. BD LSR Fortessa. A Becton Dickinson LSR Fortessa flow cytometer analyzer is being requested by investigators at Purdue University's university-wide and Cancer Center Shared  This instrument is for analysis only and has five lasers (UV, violet, blue, green and red) with 16 fluorescence detectors (two from UV laser, four from violet laser,   Creating and working with experiment in BD FACSDiva Software. • On the Browser BD FACS LSR Fortessa Standard Operation Protocol. --- Empty Waste   The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be affordable  488nm Laser (Blue) DetectorFilterMirrorDye A 710/50 685 LP PerCP, PerCP-Cy5 .5 B 530/30 505 LP FITC, GFP, CFSE, Alexa Fluor 488 C 488/10 - SSC 561nm  We have a SORP BD LSR Fortessa flow cytometer.

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This machine has five lasers: Blue (488mn), Red (640nm), Violet (405nm), Yellow Green (561nm) and  "I am a heavy user of the BD Fortessa and do over 10 color flow regularly. Being in a flow core, the machine breaks down frequently and the BD repair and  The BD Fortessa cell analyzer provides the flexibility to support measurement of multicolor flow cytometry assays of up to 16 parameters at a time. The FCF is  If it is empty replace and continue with “Fortessa run dry section”. 7) Unload tube and check if you see drops appearing at the sample needle tip (from backflush). •   27 Sep 2014 BD FACSCanto II. Sample Injection BD Horizon V500, AmCyan, DAPI.

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Apoptosis was measured on an FACS analyzer (LSR Fortessa, BD Biosciences). Phenotypes analysis of cultured cells was performed on a BD FACS Array 

The Fortessa is also the instrument used for cell cycle, cell proliferation, cell membrane potential, and… BD LSRIIs and BD LSR-Fortessa have performance, capabilities, and flexibility never seen before in a benchtop analyzer. The BD LSRIIs and BD LSR-Fortessa can be configured with up to five fixed alignment lasers and the ability to detect up to 18 colors, utilizing a revolutionary optical design. BD Fortessa HTS Procedure: 1) Remove the tube of water from the SIP and connect the HTS sample coupler.